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Journal: Cell reports
Article Title: Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides
doi: 10.1016/j.celrep.2020.107578
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Penta-His antibody QIAGEN Cat#34660; RRID:AB_2619735 Anti-Mouse IgG Sigma Cat#A9044; RRID:AB_258431 Bacterial and Virus Strains All bacterial strains are listed in Table S1 This study Table S1 Chemicals, Peptides, and Recombinant Proteins D-Methionine Sigma Cat#M9375 L-Methionine Merck Cat#105707 Critical Commercial Assays Ni-NTA Agarose QIAGEN Cat#30210 Bio-Rad Protein Assay Dye Reagent Concentrate Bio-Rad Cat#500-0006 GeneJET Genomic DNA Purification Kit Thermo Fisher Scientific Cat#K0721 Qubit® dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 Nextera XT DNA Library Preparation Kit Illumina Cat#FC-131-1024 MiSeq Reagent Kit v2 (50-cycles) Illumina Cat#MS-102-2001 Deposited Data Tn-seq experiments_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623082 WGS suppressor mutants_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623074 Oligonucleotides All oligonucleotides are detailed in Tables S2 – S4 This study Tables S2 – S4 Recombinant DNA pET28b:: vc2153 -6xHis This study FC281 pET28b:: ldcA -6xHis This study FC2025 pET22b:: vca0337 -6xHis This study FC2662 pET28b:: slt70 -6xHis ( E. coli ) Espaillat et al., 2016 FC1860 pET28b:: ldtA -6xHis Cava et al., 2011 FC1168 pET28b::AHA1477-6xHis This study FC2772 pET28b::PMI1557-6xHis This study FC2773 pET28b::STM1800-6xHis This study FC2774 pBAD 33 :: ldcA- 6xHis This study FC2753 pBAD 18-Km :: ldtA- 6xHis This study FC2759 pBAD 18-Km :: murA- 6xHis This study FC2838 pBAD 18-Km :: murB- 6xHis This study FC2839 pBAD 18-Km :: murC- 6xHis This study FC2840 pBAD 18-Km :: murE- 6xHis This study FC2841 pBAD 18-Km :: murF- 6xHis This study FC2842 Software and Algorithms GraphPad Prism GraphPad Software, Inc. https://www.graphpad.com Zeiss
Techniques: Virus, Recombinant, DNA Purification, DNA Library Preparation, Software